大发

Ms. Almaguer completed her project and was awarded the Outstanding Research Award from CSTEM. She graduated with a BS in Biological Sciences and a minor in Chemistry. She will begin her PhD in Fall 2023 in the  University of Virginia Neuroscience Graduate Program.  Her Beckman Mentor was

 

Neuropeptide Y (NPY) is one of the most abundant peptides present in the central nervous system and is responsible for regulating food intake, energy metabolism, and emotional expression. Epidermal gamma delta (gd) T cells play a role in governing wound repair and homeostasis within the skin. Emerging evidence indicates that NPY could potentially play a role in serving as a transmitter between the nervous system and immune system and even within the immune system itself. The overall goal of this project was to determine whether NPY receptors are present on epidermal gd T cells to receive signals, whether epidermal gd T cells make NPY for autocrine or paracrine response, and last, whether NPY signaling induces production of chemical messengers by epidermal gd T cells.

Ms. Wagner completed her project in 2022 and was awarded the CSTEM Outstanding Graduate award and the Chemistry Outstanding Senior. She is now working on her PhD at Scripps Research (formerly The Scripps Research Institute).  Her Beckman Mentor was Dr. Robert Iafe.

Project Summary: With the support of the Beckman Scholars Program, we created a novel methodology for developing benzylic cyclic ethers using gold(I) catalysts. Benzylic cyclic ethers are a common structural motif in the synthesis of several natural and synthetic products. These bioactive structures are present in a variety of widely available pharmaceuticals, such as potent anticancer, antifungal, anti-inflammatory, antimicrobial, antitumor, and cytotoxic agents, and novel preparations using affordable methods are vital to ensuring the targets are easier to attain. Our lab has developed a novel one-pot methodology utilizing gold(I) catalysts to yield five- and six-membered benzylic cyclic ethers intramolecularly using 1,4- and 1,5-diols. An increasing body of literature is showing that gold(I) catalysts can be highly effective at synthesizing a variety of pharmaceutical scaffolds, and to our knowledge, an intramolecular gold(I)-catalyzed etherification reaction is currently unprecedented in the literature. We have achieved successful intramolecular etherification yielding 5- and 6-membered rings, with proof of concept confirmed by way of product structural determination by 1H-NMR and moderately high yields (69-80%). This method is being optimized, and further research will include an exploration of the substrate scope of the reaction, with electron-rich and -poor diols being assessed to expand the utility of the reaction. This new method can yield effective pharmaceutical scaffolds easily and quickly, making it possible for pharmaceuticals containing these scaffolds to be lower cost and easier to develop. 

Ms. Castellanos is completing her project summer 2023 and preparing applications for graduate school.  She received the Dept. of Biological Sciences Outstanding Researcher award in 2023. Her Beckman Mentor was

 

The wound healing process is comprised of four stages that include hemostasis, inflammation, cell proliferation, and remodeling of the wound site. Chemokine receptors are part of the G-protein-coupled receptor family that regulate the homeostasis of the immune system and inflammatory responses. In the skin, researchers have identified C-C Motif Chemokine Receptor 6 (CCR6) and C-C Motif Chemokine Ligand 20 (CCL20) as regulators of T cell migration into the wound site. CCR6 is a chemokine receptor that profoundly affects the immune system during the wound healing process particularly during the inflammatory phase. The expression of CCR6 has been explored on T cells within the dermis but not on the epidermal gamma delta T cells during wound repair. Gamma delta T cells assist in protecting barrier tissues by regulating keratinocyte homeostasis and proliferation during wound healing of an organism. Since CCR6 is expressed by a subset of epidermal T cells we will investigate whether the CCR6+ subset has additional functions during wound repair that include the production of inflammatory mediators such as IL-6. Using a combination of single cell RNA sequencing, histology and flow cytometry, we will explore the timing, location and extent of CCR6 expression and the functional output of CCR6+ epidermal gamma delta T cells. In conclusion, this project inspires the development of better medical treatments for chronic wounds and injuries by understanding the expression and function of CCR6 expression on gamma delta T cells at the wound site.

Mr. St.Onge recently began his project and will graduate in 2024. He has strong interests in physiology and mammal conservation. His Beckman Mentor is .